In Wicca, a chalice, as a feminine principle, is often used in combination with the Athame (ceremonial black-handled knife), as a male principle. Combining the chalice and the athame evokes the act of procreation, as a symbol of universal creativity. This is a symbol of the Great Rite in Wiccan rituals.

A chalice is also used in a Small Rite, imbibing or sacrificing appropriate liquids. Some forms of Neo-Paganism make use of chalices in their rituals as well. A chalice may be placed on an altar or on the ground. A chalice may contain wine, whiskey, water, or other liquids.